Journal: Cell Reports Medicine

Article Title: Transcriptome-guided development of a fibrosis-reversal compound reduces skin scarring and allows regeneration via mitochondrial uncoupling

doi: 10.1016/j.xcrm.2026.102821

Figure Lengend Snippet: The mode of action for FR-1’s fibrosis-reversal effects is mitochondrial uncoupling (A) RT-qPCR analysis of COL1A1, COL3A1 , and ACTA2 in human keloid fibroblasts after treatment with different PKC family inhibitors ( n = 3). (B) Mitochondrial membrane potential detection (TMRE) in keloid fibroblasts treated with increasing concentrations of FR-1 (scale bars, 200 μm, n = 4). (C) Mitochondrial uncoupling morphology detection and puncta quantification in human keloid fibroblasts treated with increasing concentrations of FR-1 (scale bars, 10 μm, n = 2). (D) ATP/ADP mass spectrometry detection following treatment of keloid fibroblasts with increasing concentrations of FR-1 ( n = 3). (E) Seahorse XF analysis of real-time oxygen consumption rate (OCR) profiles in fibroblasts treated with FR-1 or DMSO ( n = 4). (F) RT-qPCR analysis of fibrosis biomarkers in human keloid fibroblasts after treatment with different mitochondrial uncoupling compounds, FCCP and BAM15 ( n = 3). (G) Mitochondrial membrane potential detection in keloid fibroblasts treated with different mitochondrial uncoupling compounds (scale bars, 200 μm, n = 4). (H) RT-qPCR analysis of fibrosis biomarkers in human keloid fibroblasts after overexpression of mitochondrial uncoupling proteins ( n = 3). Data are mean ± SD; n represents the number of independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, not statistically significant vs. DMSO or vector by one-way ANOVA.

Article Snippet: BAM15 (Mitochondrial uncoupler) , TargetMol , Cat# T14497.

Techniques: Quantitative RT-PCR, Membrane, Mass Spectrometry, Over Expression, Plasmid Preparation